Abstract
PRP (platelet rich plasma) has a beneficial effect on wound healing due to growth factors.When the bone graft was performed combination to PRP, the outcomes brought about a favorable condition in the graft area soon after operation. Early reports about PRP, the preparation of PRP dissociated from 400～500 whole blood as autologous blood. The procedure was complex and must return to transfusion of remaining blood after surgery. Recently, the importance of PRP is recognizing in bone graft and the opportunity to use PRP is increasing. Therefore, it is required to obtain a little quantity of PRP more easily. Thus, a method of a little PRP preparation was examined, and the optimal centrifugation method was selected.In this time, twice centrifuging separated 1 ml PRP from 8 ml whole blood was used for 20 volunteers, and the centrifugation method was prepared for four centrifugation groups.
As for the results, the group which contained the highest concentration of platelets was 2,400 rpm 7minutes/3,000rpm-10minutes(1st/2nd). The mean of platelet level was 119.3(±29.7)×104/μl and magnification was 4.9 in the group. It was found that the 1 ml PRP which separated from 8 ml whole blood contained either equivalent or more platelets compared with another techniques. Therefore, it was suspected that the 1 ml PRP also has a beneficial effect on wound and bone healing.
