Abstract
Xylan is the main hemicellulose in secondary xylem wall of angiosperm and has β-1, 4 bound main chain. An endo-β-1, 4-xylanese gene, which was originated in barley, was introduced into poplar with 35S CaMV promoter. Several lines of trasngenic poplar were obtained and the xylanase activities of the lines were much higher than that of the wild type. The result of methylation analysis showed that the amount of xylan was significantly less than that in wild type. Not so much as xylan, the transgenic poplar contained less lignin than the wild type. Moreover, fluorescent microscopic observation suggested that the lignification of interfascicular xylem in the trasgenic would be later than that in the wild.
