Abstract
The aim of this study was to qualify and differentiate between the new bones formed around hydroxyapatite-blasted implants and the cortical bone.
Evaluation of bone substance was carried out using non-decalcified tibia after 1, 2 and 4 weeks implantation by polarized light microscopy, parametric X-ray radiation (PXR) and position sensitive proportional countermicro-X-ray-diffractometer (PSPC-Micro-XRD).
Several parts of remarkably high birefringence in the new bone were revealed by the polarized light microscopy. The new bones formed around implants were compared among 1, 2 and 4 weeks after implantation: the volume of the new bone increased with time, and the Haversian lamella appeared after 4 weeks. In PXR,the cortical bone was generally observed to be radiopaque. The attached layer of the newly formed bone covering the implant was observed to be radiopaque, and its radiopacity was generally lower but similar to that of the compact bone. The full width at half maximum (FWHM) value of apatite (Ap) 002 in micro-XRD charts showed that crystallinity of the new bones increased from 1 to 2 weeks and slightly increased from 2 to 4 weeks. In this study, polarized light microscopy revealed the presence of new bones which were hardly shown by PXR. PXR showed calcification of new bone. Thus, the new bones formed around implants can be evaluated more exactly by combining these three analytical techniques.
