Abstract
Orthopedic cases of bone fracture have been treated by pulsing electromagnetic fields (PEMFs) which has the enhances quick healing. This study was to observe the cell proliferation and differentiation found in PEMFs to better understand its possible value in dental procedures.
The MC3T3-E1 cells were inoculated intoφ35 mm plastic dishes containing 2 ml α-MEM supplemented with 10% fetal bovine serum at 37℃ in a humidified atmosphere of 5% CO2. The medium was changed every 3 days. The cells were exposed to vertically directed PEMFs by placing the culture dishes between the φ70 mm helmholtz coils.
Results of the study indicated the following:
1. The best parameter of stimulation effect on DNA synthesis was 3 gauss, 25 μsec,100 Hz. The incorporation of 3H-thymidine increased the control cultures not exposed to the fields by up to 92% (p＜0.001,t-test).
2. As compared to the controls, the DNA content in the stimulated samples had a significant 126% increase (p＜0.001, t-test) after 3 days and 36% increase (p＜0.05, t-test) after 6 days.
3. The best parameter of stimulation effect on alkaline phosphatase (ALPase) activity was 3 gauss, 25 μsec, 100 Hz. ALPase activity increased the controls by up to 28% (p＜0.01, t-test).
4. Exposed cells and controls were examined by phase-contrast micrograph. The cells did not show a considerable change in morphology.
From the above results, PEMFs (3 gauss, 25 μ sec,100 Hz) stimulation promoted osteogenic cell proliferation and differentiation. On the basis of these results, I feel that this treatment can be used in the field of dentistry.
