An anchorage period in jawbone of implant material is very important for the treatment needing oral implants, and it appears to be considerably effective during the convalescing period. The author suggested that the period was shortened by pulsing electromagnetic fields (PEMFs) which was used in the treatment of pseudoarthrosis in orthopedic cases. However, the effect of PEMFs on an osteogenic cell cultured on the implant material surface was not investigated quantitatively. The purpose of this study was to obtain the quantitative measurement for effects of PEMFs on osteogenic cell. Cells were placed on the three kinds of implant materials (singlecrystal aluminum oxide, polycrystal aluminum oxide, titanium) and then the cells were cultured by PEMFs.
The Osteogenic cell which was used in this study was the MC3T3-E1 cell line. Cells were placed as 6 × 10
3 cells on the implant materials, and cultured in 2 ml of α-MEM supplemented with 10%fetal bovine serum and 60 μg/ml of kanamycinesulfate. Cells were maintaind at 37 ℃ in a fully humidified atomosphere of 5% CO
2 in air. The medium was changed every 3 days. Cells were exposed to vertically directed PEMFs (3 gauss, 25 μsec,100 Hz) by placing the culture dishes between the helmholtz coils. Experimental cultured samples were continuously exposed for 5, 7, and 9 days.
In this study, the findings can be concluded as follows:
1. The PEMFs stimulated for 3 gauss, 25 μsec, 100 Hz to the clone MC3T3-E1 cells on these implant materials were DNA and protein content. This suggests that PEMFs stimulation promoted osteogenic cell proliferation on the implant materials.
2. The result of 1. revealed that these implant materials were not inhibited and the effect was to promote cell proliferation by PEMFs.
3. The clone MC3T3-E1 cells on these implant materials were increased for alkaline phosphatase activity and hydroxyproline content. This suggests that PEMFs stimulation promoted osteogenic cell differentiation.
4. This study indicated that a titanium material had an inclination for further promoting cell proliferation and differentiation than a singlecrystal and polycrystal aluminum oxide material.
PEMFs stimulation promoted ostogenic cell proliferation and differentiation on the implant materials.
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