Abstract
Establishment of the cell line with highly lymph node metastatic potential is necessary for analysis of the mechanism of lymph node metastasis of squamous cell carcinoma of the tongue. We succeeded in establishment of oral squamous cell carcinoma cell lines with highly lymph node metastatic potential by repetition of orthotopic implantation to the tongue of the nude mice using human oral squamous cell carcinoma cell line (SAS, BSCC) . We also report some results of the analysis for characterization of these cell lines.
Two cell lines with higly lymph node metastatic potential showed 100% of transplantation ratio and metastasis to the cervical lymph node ratio, and their ability which are growth, random migration, invasion were sytronger than the original cell lines, and especially, accentuation of invasion ability was remarkable. Though in immunof luorescence antibody method, E-cadherin, β-catenin, FAK were also slightly positive in either cell line, the difference of the localization was not clear. In the analysis by the Western blotting method, there was the increase of expression level on both FAK, paxillin in the high metastatic cell line. The phosphorylation protein near 125 kDa was increased.
From the above fact, It was shown that high invasion ability, expression increase level of signal transduction molecule such as FAK, paxillin, increase the protein phosphorylation was important so that the cancer cell may acquire metastatic ability to cervical lymph node.
