In vitro inhibitory effects of various antimicrobial and antifungal agents on biofilm formation of microorganisms isolated from odontogenic infections

Abstract

The biofilm formation ability of various bacteria and Candida species isolated from patients with odontogenic infections which were grown on a microtiter plate was evaluated by the crystal violet staining method. As a result, strains with a strong biofilm formation ability were detected in some isolates of Prevotella intermedia, Porphyromonas gingivalis, Actinomyces odontolyticus, Streptococcus mitis, Candida albicans and Candida glabrata.
The inhibitory effects of various antimicrobial agents or antifungal agents on biofilm formation of these isolates were investigated at subinhibitory concentrations (sub-MICs). The inhibitory effects of linezolid, azithromycin and clarithromycin on biofilm formation were obtained at the sub-MICs for P. intermedia and P. gingivalis, but not obtained at those for other bacterial species. On the other hand, the biofilm formation of C. albicans and C. glabrata was inhibited at the sub-MIC of micafungin.