Combined effects of FGF-2 and interconnected porous hydroxyapatite ceramics on cell proliferation.
2020 Volume 39 Issue 3 Pages 121-126
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2020 Volume 39 Issue 3 Pages 121-126
PURPOSE：The repair of bone defects has received much attention in dental implant treatment and orthopedics. In recent years, fibroblast growth factor-2（FGF-2）and hydroxyapatite（HA）ceramic has been shown to enhance bone regeneration；therefore, we prepared a composite of FGF-2 and interconnected porous calcium hydroxyapatite ceramics（IP-CHA）for bone tissue engineering. The scaffolding role of FGF-2/IP-CHA granules in an MC3T3-E1 cell population and in osteogenic differentiation was investigated in vitro. The purpose of this study was to evaluate the in vitro combined effect of FGF-2 and IP-CHA ceramic on the bioactivity of osteoblasts and initial bone differentiation.
MATERIALS & METHODS：MC3T3-E1 cells were treated with FGF-2/IP-CHA, saline/IP-CHA or FGF-2 and measured using assays for alkaline phosphatase（ALP）activity and cell proliferation.
RESULTS：FGF-2 and FGF-2/IP-CHA increased cell proliferation on day 4 compared to saline/IP-CHA；however, this difference did not reach statistical significance. On day 7, a significant difference was observed between FGF-2/IP-CHA and saline/IP-CHA, FGF-2 and FGF-2/IP-CHA. Increased ALP activity was observed from day 7 to day 21 in all groups, although there was no significant difference between groups
CONCLUSION：FGF-2/IP-CHA may be clinically applicable as a novel method for bone regeneration.
