2021 Volume 40 Issue 3 Pages 65-71
This study examines the cell proliferation and collagen-producing abilities, alkaline phosphatase （ALP） activity, and the production interleukin （IL）-6 and IL-8 of the nutritional functional food “Osteo AG” in a basic experimental system using Saos-2, a human osteoblastic osteosarcoma cell line. Cell proliferation was determined by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） assay；DNA synthesis by bromodeoxyuridine-incorporation. Production of collagen, IL-6, and IL-8 was analyzed by ELISA while ALP activity by a commercial assay kit. We found that cell proliferation, production of collagen type Ⅰ, and ALP activity increased in the presence of Osteo AG. Although lipopolysaccharide of the periodontopathic bacterium Aggregatibacter actinomycetemcomitans induced production of the aforementioned inflammatory cytokines, Osteo AG inhibited this effect. In conclusion, our study suggests that Osteo AG has bone-forming ability and anti-inflammatory effects in Saos-2.
