Abstract
It was reported that some of the non-steroidal anti-inflammatory drugs (NSAID) inhibited arachidonic acid synthesis or suppressed active oxygen production to control inflammations in vivo.
We investigated the effects of NSAID on LTs generation and O-/⋅2 production of polymorphonuclear leukocytes (PMN) separated from human peripheral blood. O-/⋅2 production was detected by CLA-dependent chemiluminescence method and LTs were measured by RP-HPLC. PMN was stimulated by opsonized zymosan (OZ), myristate acetate (PMA) and Ca ionophore A23187 successively separately. Loxoprofen sodium (Loxonin®), Diclofenac sodium (Voltaren®) and Indomethacin (Indacin®) were used as NSAID. The results were as follows :
1) PMN generated only LTB4 when they were stimulated by Ca ionophore A23187. Three units of NSAIDs at the concentration of 25 μg/ml inhibited the generation of LTB4.
2) O-/⋅2 generation by OZ stimulating PMN was not influenced by NSAID. When PMA was used as a stimulant, Loxonin® suppressed the O-/⋅2 generation. But Voltaren® and Indacin® slightly enhanced O-/⋅2 generation. On the contrary, when Ca ionophore A 23187 was used as a stimulant, Voltaren® and Indacin® showed inhibitory effects on O-/⋅2 generation.
These results suggested that NSAID imposed different effects on the function of PMN.
