Effect of Aqua-Oxidizing Water for Root Surface Demineralization on Fibronectin Adsorption

Abstract

The purpose of this study was to determine the effect of aqua-oxidized water (aow) for root surface demineralization on fibronectin adsorption. Root segments prepared from non-periodontally-diseased teeth were thoroughly planed to remove cementum, and were divided into 5 groups (control, aow dipping treatment, aow varnishing treatment, citric acid dipping treatment, citric acid varnishing treatment) . The morphology of the root surface following each treatment was observed by scanning electron microscopy (SEM) . Fibronectin adsorption was evaluated by quantitative analysis using ¹²⁵I Fibronectin.
The results were as follows :
1. SEM observation indicated that root surfaces of the control group formed a smear layer, whereas aow varnishing treatment and citric acid varnishing treatment removed the smear layer and exposed dentinal tubule. Aow varnishing treatment has effective root demineralization.
2. Aow varnishing treatment and citric acid varnishing treatment had a significant increase in fibronectin adsorption as compared with the control, but there was no significant difference among the 2 other groups.
These results suggested that root demineralization by aow root treatment is useful for clinical application of fibronectin in periodontal surgery.