Abstract
Hard oxidized water is obtained through electrolysis of water with a small amount of sodium chloride. We examined the bactericidal effect of hard oxidized water, to investigate the possibility of using hard oxidized water and an ultrasonic device to wash a toothbrush. The results revealed that hard oxidized water had a strong bactericidal effect for anaerobic bacteria on a toothbrush. Moreover, it was revealed that use of the ultrasonic device increased the bactericidal effect. The findings suggest that hard oxidized water is useful for washing a toothbrush.