Abstract
Assaying the concentration of peroral antibiotics in saliva has recently been applied in TDM (Therapeutic Drug Monitoring) . The saliva level of peroral antibiotics generally profiles the different types. When high concentrations are obtained in saliva, the saliva level can be assayed simply without any preparation or with only a deproteinization. However, it is necessary to condense the saliva when the level profiles poorly even by a bioassay or HPLC (High Pressure Liquid Chromatography) . Either the evaporating method or the freeze-drying method is generally applied in the saliva condensation. In this study, the efficiency of evaporating condensation and freeze-drying condensation were compared, when the saliva level of antibiotics shows poor. The saliva and blood from 5 volunteers were tested using new Quinolone T-3262 as antibiotics. In this experiment, a new method in which were paper disks infiltrated into the test saliva prior to the evaporating or freeze-drying condensation was tested, and the recovery rates using both methods were compared.
The results obtained were as follows,
1) The preparation of soaking disks in the test saliva was convenient during manipulation, and a high recovery rate was obtained.
2) The concentration in saliva showed a close correlation with the blood concentration.
3) The recovery rate amounted to 93.6% using the evaporating method and to 67.3% using the freeze-drying method.
Conclusion: The evaporating method was shown to be superior to the freeze-drying method.
