The study on physiologically vasoactive substances extracted from skeletal muscles of fur seal

I. Determination of the method for extraction of the substances

Abstract

We have confirmed previously that physiologically active peptide-like substances extracted from the skeletal muscles of fur seals have peripheral vasodilating properties and show beneficial effect on inflamed periodontal tissues. To investigate what kinds of reliable constituents in the extracted substances exhibit the vasodilating activity, following extractive steps were tested. The first step was to determine the procedure for the extractive efficiency of the substances from the muscles of fur seals ; and the second was to detect the method of fractionating the active substances and of collecting the fractionated substances from crude extract. The vasoactive substances existed abundantly in the fraction of the molecular range less than 1, 000 M.W., but not in that more than 1, 000 M.W. As for fractionating method, utilization of Amberlite XAD-2 resin absorptive chromatography was the best way to separate the high active fraction without a loss of vasoactive activity from crude extract mixture containing high molecular- and low active-fraction.