Abstract
It has been reported that fraction I, still including an effective substance for the improvement of peripheral circulation and being non-adsorptive to resin, is fractionated by Amberlite XAD-2 resin chromatography from crude mixture, which is extracted from skeletal muscle of fur seals. Furthermore, fraction I was divided broadly into 4 fractions of A to D in compliance with electrical density in the ion exchange chromatography in this study. In each fraction, the extraction, the isolation and the identification of the substituent was investigated precisely in chemical and biological level. Results as follows:
1) Fraction-A produced following actions which were a marked aggregative action in human platelet, increasing action in peripheral blood flow and temporary endothelium dependent relaxing action in isolated vessels. HPLC analysis identified the vasoactive substance in fraction A with 5′ ADP.
2) As for fraction-B, a marked vasodilating action and inhibitory action of human platelet aggregation were observed. This active substance was identified to be 5′ AMP by HPLC analysis.
3) A vasoactive action of the fraction-C was faint and it was chromatically confirmed to be containing carnosine and anserine in Fr. C.
4) Fraction-D showed the strongest increase of a peripheral blood flow in comparison with any other fraction and produced a powerfull contraction in isolated vessels. This vasocontractive action was inhibited by antihistamine and H1 receptor-antagonist so that it was considered to be caused by histamin like substance. The active substance was identified finally to be histamine by HPLC analysis.
