Abstract
Limulus positive substances in Dermatophagoides farinae (Df) extracts were studied. Df antigens were extracted by acetone precipitation according to the method of Le Mao et al. Limulus positive substances were detected in crude Df antigen, Df50d and Df80d. The weights of limulus positive substances were estimated to be approximately 10%, 1% and 0.01% of the protein content respectively. The limulus positive substances were eluted at void volume using Sephadex G-100 column Gel-filtration.
