Abstract
Sucrose clearance was measured after sucrose loading to compare the results of three different loading concentrations and two sampling methods. In seven adults,5%,10% and 20% sucrose solutions were given and changes in the sucrose, lactate, and glucose were measured over time using two sampling techniques, the spitting method and the spot method. An enzyme electrode method capable of measuring samples on the microquantity level was utilized. The following conclusions were drawn:
1. The sucrose concentration changed in a biphasic way using both the spot and spitting methods. The spot method tended to yield higher sucrose readings. Increasing the load concentration resulted in delayed sucrose clearance.
2. The lactate concentration showed in initial increase followed by a decrease. The peak persisted for 5-7 minutes. The spot method appeared more reliable as it showed more pronounced changes as the load concentration was increased.
3. The glucose concentration changed in a similar fashion using the spot or spitting methods.
4. Using the spot method, the relationships among the sucrose, lactate and glucose after loading were more clearly shown than by using the conventional spitting method.
5. For sucrose clearance measurements utilizing techniques capable of measuring samples on the microquantity level, sampling using a spot technique appears easier and more accurate than using the conventional spitting method.
