Purification of LDH Isozyme from Pig Dental Pulp

Abstract

Lactate dehydrogenase (EC.1.1.1.27; LDH) catalyzes the conversion between pyruvate and L-lactate as the last step of the glycoytic pathway. We devised methods for the separation and purification of dental pulp LDH isozyme from the first molar (M1) of pigs.
LDH-1 isozyme was purified to a specific activity of 442.2 units/mg of protein, representing an approximately 1580-fold purification from the crude extract, using DEAE Sephadex A-50 column chromatography and Blue dextran affinity chromatography. LDH-2 and 3 isozyme were also highly purified and high recovery.