Abstract
The prognosis for replantation of traumatically avulsed teeth is decided by the periodontal ligament (PDL) cell condition. In other words, the highest level of PDL cells brings good results without ankylosis or replacement resorption of the root. If the patient cannot obtain the most ideal solution for storage, the tooth must be temporarily placed in a solution that is most readily available and then it has to been carried to a dental clinic. The purpose of this study was to compare the viability of cultured human PDL cells in readily available storage media.
Five solutions including milk, saline, three contact lens solutions for soft contact lens were used as experimental solutions in this study. MEM including 10% FCS was used as the control medium. The PDL cells were cultured from healthy extracted human teeth.
After PDL cells were cultured for 8 days, they were exposed to each solution for 15 minutes at 4r. The MTT assay was measured immediately after exposure and subsequently followed at 1,2,3,5and 7 days after exposure. The morphological changes of the PDL cells were observed immediately and on 7 th day after exposure.
The results were as follows.
1. The growth activity: Milk showed superior activity in the solutions. Saline and three contact lens solutions showed good activity.
They showed, however, lower activity than milk. The activity of each contact lens solution was a different type of activity.
2. The morphological changes: Milk caused healthy cells with spindle-like morphology. In the saline and three contact lens solutions, the cells appeared rounded and shriveled at immediately after exposure, but recovered their original appearance 7 days after exposure.
These results suggest that contact lens solutions are inferior to milk used as first aid when teeth avulse. They are however useful solution as an emergency preservation for short term and in cold storage.
