運動後尿Donaggio反応陽性物質の同定
1978 年 27 巻 3 号 p. 117-123
詳細
1978 年 27 巻 3 号 p. 117-123
Attempt has been made to identify Donaggio positive substances in urine after exercises (severe running) . Results obtained were as follows;
1) The substances having the high titer of Donaggio reaction, were probably derived from glycoproteins, which include α1-globulin, and small amounts of α2-globulin, by cellulose-acetate electrophoresis.
2) This α1-globulin nearly composed of α1-acid-glycoprotein (so called α1 AG) and other α1-glycoprotein were not found, namely tryptophan poor α1-glycoprotein (α1 T), α1-anti-trypsin (α1 AT), and α1-anti-chymotrypsin (α1X) by immuno double diffusion. One of the α2-globulin on Donaggio positive substances was found to be α2HS-globulin (α2HS) . Single radial immunodiffusion (SRID) and electro-immunodiffusion (EID) were used to measure the levels of Donaggio positive substances (α1AG and, α2HS) in urine before and after exercise, levels of α1AG, α2HS in urine measured by SRID were almost similar to those by EID.
3) Both α1 AG and α2 HS in exercised urine were slightly decreased heating (100°C 10 minutes) after, being acidified with acetic acid (pH 5 or pH 4) . The lower pH indicated, the lower α1 AG and α2 HS level.)
The treatments were (only boiled and filtrated) described by Donaggio in his original manuscript. Data indicates that adjusting urine to pH 5 is recommended.
