抄録
The purpose of this studies is to investigate the Donaggio reaction positive substances in serum, by the same method as urine.
'Obstacle phenomenon for precipitation of methylen blue and ammonium molybdate' had been also observed to be positive in serum.
Under the influence of stress (not only strenous physical exercise but also rest state, derived from the mainly catabolating of connective tissue), these substances had been guessed to pass into blood stream and thereafter into the urine.
Donaggio titer in serum solution only could not be measured by Sato's standard method, because of coagulation and impossibility of precipitation on deproteinization. It was found to be able to measure by mixing urine to serum.
Results obtained were as follows
1) Diluted serum, by mixing of utrafiltrated urine, kept high level of Donaggio titer.
2) Donaggio titer in serum decreased by deproteinization at pH. 5, especially by heat 100°C for 10 minutes.
3) Semi-quantitative isolation were performed by means of electrosyneresis, α1 acid glycoprotein (α1-AG), α1-anti trypsin (α1-AT), α1-anti xymotrypsin (α1-X) in α1 globulin region and Zn binding α2-glycoprotein (Zn-α2), α2 HS-glycoprotein (α2-HS) in α2 globulin region were identified as Donaggio positive substances from deproteinized serum.
Furthermore, identity of Donaggio positive substances between serum and urine must be examined by immunological technique, for example-line-immunoelectrophoresis.
