A TLC-Based Identification Test for the Crude Drug Angelicae Dahuricae Radix

Abstract

A TLC-based method was developed for the identification of the crude drug Angelicae Dahuricae Radix (Angelica Dahurica Root; root of Angelica dahurica Bentham et Hooker filius ex Franchet et Savatier). UV-sensitive constituents in 11 samples of Angelicae Dahuricae Radix available on the market in Japan were compared using TLC. Six common spots were detected in all samples and these were isolated and identified as isoimperatorin，phellopterin，imperatorin，byakangelicol，oxypeucedanin hydrate, and byakangelicin based on the results of spectroscopic methods. Among them, two compounds, oxypeucedanin hydrate and byakangelicin, were concluded to be characteristic of Angelicae Dahuricae Radix. The present data could be useful for the development of convenient TLC tests for identifying Angelicae Dahuricae Radix.