Abstract
A cell capturing system has been developed to isolate particular cells in the limited area of corresponding organs, and is, therefore, a strong tool for analysis on the profiles of gene expression in individual cells. In order to establish the procedure for efficient isolation of rice genes which are expressed in phloem tissues and to analyze the profiles of gene expression of phloem tissues, this system was applied for isolation of these tissues. RNA was extracted from these cells, and used for construction of a specific cDNA library. Sequence analysis of 413 randomly chosen clones from the library revealed that the clones could be subclassified into 124 different groups that contained related sequences. Approximately 37% of the groups had novel components while approximately 63% were homologues to the known genes. The results of gene expression analysis of the cDNA clones are discussed.
