Abstract
Most proteins in chloroplast are synthesized in the cytosol, and are imported into chloroplast through the protein import complex. Several components of the complex have been identified in pea. They are named Toc(translocon at the outer envelope membrane of chloroplast) and Tic(translocon at the inner envelope membrane of chloroplast). Both homologues are encoded in Arabidopsis genome. We isolated #2774 mutant that shows pale green phenotype from Arabidopsis tag line made by Feldmann. This mutant was identified as a Tic40 mutant. The one-two weeks old plant is pale green, but three-four weeks old plant is green. To investigate the accumulation of proteins and mRNA of photosynthesis component, we tested Western blot and Northern blot analyses. These analyses indicated that mRNA levels encoded in Arabidopsis genome were higher in mutant than in wild type, but protein levels were reversed. We will report the role of Arabidopsis Tic40 in chloroplast development.
