Abstract
We isolated an Arabidopsis chloroplast mutant #2755 from Feldmann T-DNA tag line, which could not grow photoautotrophically. The mutant could grow on agar media with sucrose but its phenotype was pale green and growth rate was very slow compared with wild type. Analysis of segregation of phenotype confirmed that the mutant was disrupted by T-DNA insertion. Amino acid sequence of T-DNA inserted gene was highly homologous to that of DHNA-prenyltransferase, an enzyme of the biosynthetic pathway leading to phylloquinone of Synechosystis sp. PCC 6803. Phylloquinone is the primary electron acceptor of photosystem (PS) I. The data of PAM (Pulse Amplitude Modulation) indicated that electron transport rate in PS I of the mutant was decreased. To investigate the accumulation of proteins and mRNA of photosynthesis component, we tested Western blot and Northern blot.
