Abstract
The plant cell wall is a dynamic architecture constructed by many kinds of proteins, most of which are encoded by multi-gene families. Although the complete sequencing of Arabidopsis genome has revealed a large number of putative cell wall related gene, little is known about the whole aspect of proteins committed to the cell wall formation. In order to investigate the profiles of cell wall related proteins, we have generated the protoplast from Arabidopsis suspension cultured cells, and analyzed a whole complement of proteins localized in the cell wall during the process of the cell wall regeneration by 2D-PAGE. Mapping of these proteins have shown remarkable changes in proteins extractable from the cell wall during the process of cell wall regeneration, particularly those with high pI. We are now sequencing these proteins by MALDI-TOF/MS to identify genes required for each step of the cell wall formation.
