Abstract
Plant cell wall, which determines the shape of cell, is built and maintained by actions of proteins encoded by many multi-gene families. Comprehensive expression analysis for cell wall proteins is required for understanding the mechanism of modification of cell shape. We have developed an experimental system by which we can alter shape of Arabidopsis suspension-cultured cells. When incubated in a medium containing 2,4-D (2,4-dichlorophenoxyacetic acid), no cell elongation was observed in the cells. Upon transfer to a medium containing IAA (3-Indole acetic acid) and IPA (isopentenyladenine) from the 2,4-D medium, cell elongation was induced. We compared expression profiles of cell wall proteins between the elongated cells and normal cells. We found proteins localized in the cell wall changed notably as the cells elongated.
