Abstract
The chloroplast characteristic of plants has photosynthetic activity and develops in restricted organs such as leaves and stems. To reveal the mechanisms of building up chloroplasts in an organ-specific manner, activation tagging was applied to a parental line transformed with hygromycin B hopsphotransferase gene under the control of RBCS-3B promoter, resulting in selection of ces (callus expression of RBCS) mutants. The calli of ces101 were green, where transcripts for RBCS were approximately 700 times higher and those for SIG1 encoding σ_factor responsible for transcription of chloroplast photosynthesis genes were also higher in comparison with those in the parental line. A receptor-like kinase gene closed to the integrated T-DNA was driven by the enhancer sequences in transformants, which exhibited the same phenotype as ces101 line, leading the conclusion that the CES101 is the receptor-like kinase. We would also report results of the analysis of ces102 line.
