Analysis for Methyl Jasmonate-specific Expression Changes in Arabidopsis by cDNA Macroarray

Abstract

Jasmonates are widely distributed in the plant kingdom, and modulate wounding responses, disease responses, and anther development. Although various kinds of jasmonates were found in plants, specific roles of each jasmonate are still unclear. Using cDNA macroarray, we identified jasmonic acid (JA)-, methyl jasmonate (MeJA)-, and 12-oxo-phytodienoic acid-responsive genes. JA biosynthesis genes such as LOX2, AOS, AOC, OPR1, and OPR3 are induced by all treatments, however, these genes showed different expression profiles in each treatment. To focus on the MeJA function, we identified jasmonic acid carboxyl methyltransferase (JMT) mutant from a collection of Ds transposon insertion lines made in RIKEN. When jmt was treated by JA, the expression of LOX2 was altered, wheares other JA biosynthesis genes were not changed. In this presentation we will report cDNA macroarray analysis for jmt.