Abstract
To understand molecular mechanisms underlying temporal and spatial regulation of vascular pattern formation, which has provided a model for studying pattern formation and tissue differentiation in plants, we have been screening genes involved in vascular formation using Arabidopsis gene trap lines established in Kazusa DNA institute. We screened 93 lines having GUS staining along with vascular network in leaves from 47800 gene trap lines. We are now trying to determine GUS insertion locus using methods of TAIL-PCR and/or 5RACE. In the 30 lines, which have GUS staining pattern specific to vascular systems, KG09040 showed fragmentation of lateral vein, which is a typical phenotype of van mutants, indicating that the KG09040 gene may be responsible for provascular tissue patterning. Here we report histochemical and molecular genetic analyses of KG09040 and some other gene trap lines.
