Monitoring of 12-oxo-phytodienoic acid -responsive genes in Arabidopsis by cDNA macroarray

Abstract

Jasmonic acid (JA) is a cyclopentanone derivative that originates from linolenic acid via an octadecanoid pathway consisting of several enzymatic steps. JA regulates diverse processes such as wound responses, disease responses and pollen maturation in plants. A recent report suggests that a cyclopentenone precursor of JA, 12-oxo-phytodienoic acid (OPDA), can also regulate wound induction of genes. However little is known about the difference in the genes which response to JA and OPDA. We screened JA- and OPDA-responsive genes separately using cDNA macroarray covering about 9000 genes of Arabidopsis. Most of JA- and OPDA-responsive genes are induced by both treatments. However we identified genes which specifically responded to OPDA or JA. Differential effects of JA and OPDA will be discussed in the presentation.