Abstract
The PAL gene (gDcPAL3) plays the most important role in the induction of anthocyanin synthesis in carrot suspension cultured cells regulated by 2,4-D. Deletion analysis of the gDcPAL3 promoter indicated that the promoter region between -258 and -194 were required to give full activity. This region contained the GCC sequence, which has been known as the cis-element of the ERF family having the plant specific DNA binding domain, AP2/ERF domain. We isolate two AP2/ERF transcription factors, DcERF1, DcERF2, that interact with the GCC sequence of the gDcPAL3 promoter using yeast one-hybrid system from two cDNA libraries, one of which was prepared from carrot cells of the normal line induced for anthocyanin synthesis in the medium lacking 2,4-D, and the other was done from the cells of the mutant line in which anthocyanin was continuously synthesized in the medium containing 2,4-D. Binding activity and expression patterns of them will be reported.
