Isolation and Properties of a cDNA for a Transcription Regulatory Factor of Carrot Phenylalanine Ammonia-lyase Gene (gDcPAL1)

Abstract

Phenylalanine ammonia-lyase (PAL) acts as the key enzyme in regulating metabolic flow from the primary metabolism to phenylpropanoid metabolism, and the genes encoding PAL are regulated by both environmental cues. It has been revealed that the expression of gDcPAL1 gene in suspension-cultured carrot cells is induced by dilution effect, elicitation and UV-B irradiation, and that L1- and L5- boxes in the gDcPAL1 promoter play an important role to the regulation of gDcPAL1 gene expression. We isolated a cDNA for the transcription regulatory factor being highly similar to MYB protein, which binds to L1-box or L5-box using yeast one-hybrid system. We will report the features of DNA-binding activity and specificity of this MYB protein in vivo and in vitro.