Abstract
Recent advances of molecular biological studies on gibberellin (GA) biosynthesis and signaling have succeeded to isolate some GA-related genes. Consequently, we can take new approach to know where bioactive GA is produced and GA is perceived through analysis on the expression pattern of GA-biosynthetic and GA-perception genes. For detecting the expression of these genes, we analyzed using promoter-GUS transgenic plants or in situ hybridization. Expression of the genes we used as marks was observed in young developing leaves, apical region of roots and elongating stems. At the inflorescence and floral stage, expression of the genes was observed in the shoot meristems just before flowering, floral meristems, stamen primordia and tapetum cells. The overlapped expression of GA-biosynthesis and GA-perception genes in these various tissues and organs indicates that active GA-biosynthesis occurs at the same site of GA-perception. This work was in part supported by PROBRAIN.
