Abstract
We measured fluorescence and absorbance spectra of single heterocyst and vegetative cells of Nostoc sp. KU001 Cells of Nostoc sp. grown under different light conditions show different pigment composition due to complementary chromatic adaptation.
Absorption and fluorescence spectra of intracellular pigments are scanned at room temperature by high-resolution laser microspectrometry using a confocal laser microscope coupled with a cooled CCD (for spectral measurements) and cooled PMT (for imaging). Spectral features are measured with spatial and wavelength resolutions of 200nm and 1nm, respectively.
The technique revealed different pigment composition and florescence yield in each cell and gives new information for the range of cell phenotype wider than that obtained by conventional macroscopic measurements.
