Establish of ARAMAP system that is a rapid mapping technique to identify the mutated gene(s) in Arabidopsis thaliana.

Abstract

Map-based cloning techniques are widely used to identify the mutated gene(s) in plant, because chemical agents, such as EMS, are generally used to induce mutations. However, map-based cloning was used to be time consuming to identify the mutated gene(s).

Whole genome sequence information has already been opened in Arabidopsis, we established a rapid and high throughput mapping technique, ARAMAP system, based on the information of single nucleotide polymorphism (SNP). To demonstrate the accuracy of this system, we performed SNP-based cloning for a floral internode mutant that we isolated from T-DNA tagging line. Although this mutant is well linked with T-DNA insertion, by using this system we could map to the same locus within two days in a region between 8.559Mb and 13.13Mb of a chromosome. We finished setting of functional markers on the five chromosomes of Arabidopsis in a interval of 0.26Mb to be able to fine mapping of mutant.