Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 44
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Linkage analysis using RAPD markers in Chinese cabbage (Brassica rapa, syn. campestris).
*Norihiko NakazawaHiromitsu NakanishiGoro TaguchiMineo KojimaEtsuo MatsumotoNobuaki Hayashida
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Pages 568

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Abstract
The improvement in qualities and production efficiency is one of the important subjects in breeding of Chinese cabbage. However, it is hard to use biotechnologies such as GMO (gene modification organisms) due to social objections to these techniques. To establish a new breeding method for Chinese cabbage other than GMO, we are constructing a linkage map using RAPD (Random Amplified Polymorphic DNA) markers and expanding it to the QTL analysis of the genes for various traits.
In this study, commercially available 36 random primers are used for PCR with combinational manner. From the total combinations(666), about 100 RAPD markers are found to show good reproducibility. Consequently, they are newly located on the linkage map of Chinese cabbage. In this presentation, we would like to report the application of this map to the QTL analysis for economically valuable traits.
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© 2003 by The Japanese Society of Plant Physiologists
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