Analysis of rice genes induced by inoculation with rice blast fungus and ACC treatment using rice 9000 cDNA microarray

Abstract

To study defense mechanism in rice (Oryza sativa L.) against rice blast fungus infection, we used 9000 rice cDNA microarray for analysis of gene expression profiling. We analyzed the effect of 1-aminocyclopropane-1-carboxylate (ACC), because we supposed ethylene signal pathway contributes the mechanism. The forth leaf of the susceptible cultivar (cv. Nipponbare) and a resistant cultivar (cv. IL7) were inoculated with rice blast fungus race 003 or treated with ACC, and total RNA were extracted at 42 hr or at 24 hr respectively, supplying to rice 9000 cDNA microarray. The result showed induced expression of 1,541 clones specifically in the resistant cultivar. ACC treatment caused enhanced expression of 51 clones. Expression of 28 clones were increased in the both treatments. The results showed that genes involved in ethylene signal pathway might be included in the genes induced by the defense response. The gene expression profiles will be shown and discussed.