Abstract
With the completion of the Arabidopsis genome sequence and the improvement of technologies to analyze gene expression and protein accumulation comprehensively, the focus of plant biology have shifted from gene identification to gene function elucidation. The changes in expression and protein profiles result in more complicated change in metabolite accumulation pattern. Hence, metabolic profiling can be a powerful tool to clarify the mechanisms of various phenomena in living cells. Our aims are to analyze non-targeted metabolic profile and to obtain new knowledge by integration of expression profile and metabolic profile.
Expression profiles of 3-week-old S- or N-starved Arabidopsis were analyzed using macroarray. Global expression profiles of S- and N-starved plants were similar to each other. Different sets of genes were regulated by nutritional stresses in leaves and roots. By statistical analysis and cluster analysis, the genes induced specifically under S or N deficiency were identified.
