Integrated studies of transcriptome and metabolome analyses of Arabidopsis under nutritional stresses II

Abstract

Using the same plant materials as reported in the former presentation (Integrated studies of transcriptome and metabolome analyses of Arabidopsis under nutritional stresses I), the accumulation of metabolites was analyzed comprehensively. By ultra-high resolution Fourier transform ion cyclotron (FT)-MS, 1,000 to 1,500 mass peaks were detected. We have developed a new program to identify compounds from accurate m/z obtained by FT-MS. Even in case that mass peaks cannot be identified, they are useful as fingerprints and hence global metabolic profile can be analyzed. As is the case with gene expression profiles, metabolic profiles of S- and N-starved plants were similar to each other. By cluster analysis, the metabolites accumulated specifically in S- or N-starved plants were identified. In this presentation we will report what was clarified by the integration of transcriptome and metabolome analyses.