抄録
In the nitrate assimilation pathway of plant, nitrite, produced after nitrate reduction by cytosolic nitrate reductase, enters into the chloroplast and is reduced to ammonium. Nitrate at a physiological concentration could be taken up to the chloroplast by an active transport. The transport of nitrite into chloroplast seems to be an important step in nitrogen metabolism in plants that use nitrate as major nitrogen source.
CsNitr1 cDNA was cloned and characterized as a plastid transporter for a nitrogenous compound. In this study, we analyzed the function of CsNitr1 by detecting quantitatively the translate using specific antibodies that were raised against hydrophilic region of CsNitr1. The expression of CsNitr1 was induced in green tissues by nitrate and nitrite only under the light depending on chloroplast integrity, suggesting that the function of CsNitr1 links with photosynthesis, especially with nitrite reduction in it.
