Abstract
A Japanese indigenous Fern (Athyrium Yokoscense) is known as a heavy metal hyper-accumulator, which ability had been utilized as an index for searching mines at the old era. So, we aimed to clarify the genetic background of the tolerance and accumulation capacity. Here, we reported the condition for the callus formation, which would be useful to analyze the ability at the stable and homogenous conditions, and result from the analysis of the cadmium tolerance and accumulation capacity at the callus stage. Calli were formed from fronds of the fern when they were placed on the MS-medium supplemented with sucrose, agar and various combinations of Kinetin and 2,4-D. Then, these calli were grown in the same medium containing some concentrations of cadmium. The callus growth was comparable to that of the mature plant even in the higher concentration of cadmium, indicating that the cell itself would contain the ability.
