Visualization of real time ⁵²Fe translocation in graminaceous plants

Abstract

We traced the time course of ⁵²Fe translocation in graminaceous plants by a PETIS (Positron Emitting Tracer Imaging System) method. In all results, ⁵²Fe was first accumulated to 'discrimination center' (DC), which is the basal part of the leaf. I) Barley plants that had been cultured with and without Fe were supplied ⁵²Fe-deoxymugineic acid (DMA) to the root. Fe deficient barley accumulated ⁵²Fe to DC about 12 times as much as Fe sufficient barley. II) The maize ys1 mutant which is defective in Fe(III)-MAs transporter 'YS1' and wild type plants were cultured without Fe and supplied ⁵²Fe-DMA to the root. In the ys1 mutant, ⁵²Fe translocation at the DC was suppressed to about 20% of that of the wild type plant.