Abstract
Tobacco BY-2 chitinase and glucanase activities were induced by the addition of the pathogenic fungus Alternaria alternata culture medium. The object of this study is isolation and analysis of the elicitor active compounds from culture medium.
The culture medium was autoclaved and filtrated to remove the fungus body. 99% methanol-insoluble fraction of the filtrated solution had elicitor activity. The activity fraction was separated by removing protein, sodium hydroxide extraction and 75% methanol extraction. Finally, the elicitor was isolated by a gel filtration chromatography. The elicitor activity was assayed by the BY-2 chitinases induction method.
The isolated elicitor was composed of carbohydrates with molecular weight of >4000. BY-2 chitinase was induced with the concentration of 200 μg ml-1. Hydrolysates were converted the alditol acetates and analyzed with a gas chromatography. As the results, the carbohydrates elicitor from A. alternata was composed of mannose, glucose, and galactose.
