Abstract
The tobacco BY-2 chitinases were induced by the addition of Alternaria alternata cultured medium. In this study, we focused on BY-2 β-1,3-glucanase, and investigated the effect of A. alternata and 12 chemical stresses on the β-1,3-glucanase induction.
BY-2 glucanase were induced by the addition of salicylic acid, laminarin and several chemical stresses, and especially, A. alternata culture medium induced glucanase activity markedly. On the other hand, methyl jasmonate and abscisic acid did not induce the glucanase. By addition of A. alternata, the glucanase activity increased after 12~24h, and reached the maximum at 60h and then decreased. The time course pattern of glucanase activity was different from the pattern of BY-2 chitinases. Now, we try to cDNA cloning of the β-1,3-glucanase.
