Abstract
When phosphate in soil is limiting, plants behave various responses to adapt this condition. However, the details of these responses are still unknown. In this study, to perform comprehensive and systematic analysis about these responses, cDNA microarray and quantitative Real-Time PCR analyses were conducted.
Rice plants grew by hydroponic culture with or without phosphate (1 ppm or 0 ppm, respectively), and then separated into shoots and roots. Total RNA was isolated and used for the analysis of gene expression. Expression of some genes related to phosphate absorption was induced by Pi-deficiency. It was confirmed that the expression of genes involving with carbon metabolism was changed in shoot under Pi starvation. On the other hand, no significant increase of expression of the genes related to organic acid exudation was observed under Pi-deficient condition.
