Abstract
Plants developed strategy to recycle phosphorus in their organ to replenish the requirement of other organ (especially newly growing organ). We focused on the role of Ribonuclease (RNase) and Acid phosphatase (APase) for the decomposition of phosphorus compound to regulate plant phosphorus level. As many phosphorus compounds are closely related to carbon metabolism, the expression level of following enzymes are evaluated by RT-PCR, Triose phosphate translocator, Phosphoenolpyruvate translocator (PPT), Phosphoenolpyruvate carboxylase, Pyruvate kinase, NAD-Glycerol 3-phosphate dehydrogenase, NADP-Glycerol 3-phosphate dehydrogenase. Under phosphated deficient condition, the level of inorganic phosphate decreased dramatically, and the enzyme activity of RNase and APase increased. The content of hexose decreased, instead starch content increased under phosphate deficiency. As the expression level of PPT increased dramatically, the carbon flow from cytosol to chloroplast as phosphoenolpyruvate may play an important role to maintain carbon metabolism under phosphate deficient condition.
