Abstract
When plants grow under low-P conditions, acid phosphatase (APase) expresses in root exudates and tissues, which contribute to hydrolyze organic phosphate compounds for P absorption and translocating into other tissues, respectively. Objective of this study is to investigate function of APase isoforms under low-P condition.
Lupin plants were grown by hydroponic culture with (+P treatment, 2 ppm) or without P (-P treatment, 0 ppm). Plants were separated to each organ. APase isoforms were separated by IEF. Various APase isoforms in roots and leaves increased by P treatment. Notable effects by -P treatment were not observed in cotyledons and stems. Only one isoform in pI 4.7 was detected in the exudate of -P roots. mRNA accumulation of three APases was analyzed. mRNAs of cell wall localized and phytate specific APase was observed in all organs. On the other hand, mRNA of secretory APase was detectable only in -P roots.
