Arabidopsis Encyclopedia using Full-length cDNAs and its Application for Functional Genomics

Abstract

Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. Using the biotinylated CAP trapper method, we constructed full-length cDNA libraries from Arabidopsis plants and isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. They were clustered into 14,668 nonredund ant cDNA groups, about 60% of predicted genes1). We have determined full-length cDNA sequences of 12,189 RAFL cDNA clones as of Nov. 29, 2002. We have also used the RAFL cDNAs for the microarray analysis of expression profiles of Arabidopsis genes under drought, cold and high-salinity-stresses2),3),4). In this meeting, overview of our RAFL cDNA project will be presented.
1) Seki et al. (2002) Science 296:141-145. 2) Seki et al. (2001) Plant Cell 13:61-72. 3) Seki et al. (2002) Plant J. 31:279-292. 4) Seki et al. (2002) Funct. Integ. Genomics 2:282-291.