Abstract
Soil salinity is one of the severe abiotic stresses for plant growth and agriculture. Recently, several salt overly sensitive (sos) mutants have been isolated in Arabidopsis. In this study, the expression profiles of sos2-1 and sos3-1 mutants were analyzed by cDNA microarray.
We regarded the genes with expression ratios (high-salinity/unstressed) greater than three times that of lambda control template DNA fragment in at least 1 time-course point as high-salinity-stress-inducible genes. We detected 347, 157, and 125 genes as high-salinity-inducible genes in wild type, sos2-1, and sos3-1 mutants, respectively. Among them, expression of DREB2A, RD29A and RD17 were high-salinity-stress-inducible in sos2-1 and sos3-1 mutants as well as in wild type. This result indicates that SOS pathway is independent of DREB/CBF pathway. The expression of sos1, a transcription factor, and some unknown protein genes were not high-salinity-stress-inducible in sos2-1 and sos3-1 mutants, suggesting that these genes are regulated by SOS pathway.
